Table 1 Binding measurements for lit- and dark-state OptoNBs.
From: Optogenetic control of protein binding using light-switchable nanobodies
Variant | Illumination | kon (μM−1 s−1) | koff (s−1) | KD (μM) |
|---|---|---|---|---|
LaM8 | None | 0.072 ± 0.004 | 0.019 ± 0.001 | 0.26 ± 0.02 |
LaM8-GG15C450V (pseudo-dark) | None | 0.039 ± 0.003 | 0.021 ± 0.001 | 0.53 ± 0.05 |
LaM8-GG15I532E,A536E (pseudo-lit) | None | 0.038 ± 0.003 | 0.12 ± 0.01 | 3.1 ± 0.3 |
LaM8-GG15 | 450 nm light | 0.038 ± 0.003 | 0.096 ± 0.002 | 2.5 ± 0.2 |
LaM8-AK74C450V (pseudo-dark) | None | 0.018 ± 0.004 | 0.52 ± 0.01 | 29 ± 10 |
LaM8-AK74I532E,A536E (pseudo-lit) | None | 0.034 ± 0.001 | 0.14 ± 0.01 | 4.1 ± 0.2 |
LaM8-AK74 | 450 nm light | 0.021 ± 0.001 | 0.16 ± 0.01 | 7.4 ± 0.5 |
