Fig. 2: Detecting transcription of Dscam1 in NB and neurons using in situ RT-PCR. | Nature Communications

Fig. 2: Detecting transcription of Dscam1 in NB and neurons using in situ RT-PCR.

From: Dscam1 establishes the columnar units through lineage-dependent repulsion between sister neurons in the fly brain

Fig. 2

a Schematic of Dscam1 gene structure and alternative splicing indicating the primers used for in situ RT-PCR. b Homophilic binding of the identical Dscam1 isoform causes repulsion. c Schematic representation of lineage depend repulsion between neurons that derive from the same NB expressing the same Dscam1 isoform. d Control PCR (green), Lsc (blue) and Dpn (magenta) on the surface of the brain in a lateral view showing the NB layer, n = 22 (see Fig. 1b). e Quantification of signal intensity in the dotted box in (d). f Control PCR (green), Lsc (blue) and Dpn (magenta) in a dorsal view showing the decrease of mRNA signal in older NBs, n = 16 (same orientation as Fig. 1b). g, h, j Lateral views showing the neuron layer (see Fig. 1b). g Control PCR (green) and Dpn (magenta), n = 47. h Intron PCR (green) and Dpn (magenta), n = 13. i Quantification of signal intensity in the boxes in (g, h). Background signal was subtracted for Dpn. jDscam1 9.1 PCR (green) and Dpn (magenta) in a lateral view showing the neuron layer, n = 10 (see Fig. 1b). k Quantification of signal intensity in the boxes in (j). Each box contains one NB. Intensities in the dotted boxes are plotted with dotted lines. Scale bars indicate 20 μm. Source data are provided as a Source Data file (e, i, k).

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