Fig. 8: Ani9 inhibits polycystic kidney disease in a mouse model for ADPKD.

a Representative images of kidneys from induced (Pkd1^−/−) mice, untreated (daily i.p. injection of corn oil) (n = 8 kidneys analyzed from n = 4 animals) or treated with Ani9 in corn oil (Ani9, 0.5 mg/kg/day, i.p.) for 30 days (n = 8 kidneys analyzed from n = 4 animals). Scale bars 1000 µm. b Cystic index (%) 8 weeks after induction of Pkd1^+/+ and Pkd1^−/− mice, untreated or treated with Ani9. Ani9 reduced cyst growth in Pkd1^−/− mice (^§P = 0.001). ^§Unpaired two-sided t test. c, d Proliferating cells identified in Pkd1^+/+ and Pkd1^−/− kidneys by the proliferation marker ki-67 (red). Scale bars 50 µm. Proliferation was upregulated in Pkd1^−/− mice (^#P < 0.0001), but was largely inhibited by Ani9 (^§P = 0.021). ^#§One-way ANOVA and Tukey’s post-hoc test. Number of ki-67 positive cells was normalized to the tissue area. Mean and error bars indicating ±SEM (25 independent images from n = 8 kidneys of n = 4 animals each). Mean and error bars indicating ±SEM. Source data are provided as a Source Data file.