Fig. 2: TBI causes changes in meningeal lymphatic vasculature morphology.

Mice received TBI or sham treatment and then meningeal whole mounts were harvested 1 week, 2 weeks, 1 month, and 2 months later. a Representative images depicting transverse sinuses (left) and meningeal lymphatic vasculature loops near lymphatic hotspots (right) and (c) quantification of the percent area coverage of Lyve-1 antibody staining and (d) the number of loops in meningeal whole mounts. Solid boxes show zoomed insets of the hotspots along the transverse sinus on the right. Red arrow heads in the insets of (a) denote meningeal lymphatic vasculature loops. b Representative images depicting meningeal lymphatic vasculature sprouts along the transverse sinuses and (e) quantification of the number of sprouts found in meningeal whole mounts. Red arrows in (b) denote meningeal lymphatic vasculature sprouts. f Quantification of the diameters of the meningeal lymphatic vessels. Each data point represents an independent mouse and is an average of 70 measurements along the transverse and superior sagittal sinuses per mouse. Data in (c–f): Sham n = 15, 1 week n = 11, 2 weeks n = 7, 1 month n = 8, 2 months n = 8; pooled data from four independent experiments. All n values refer to the number of mice used and the error bars depict mean ± s.e.m. P values were calculated by a one-way ANOVA with Dunnett’s multiple corrections test (c–f). mo month(s), mpi month(s) post injury, wk week(s), wpi week(s) post injury. Source data (c–f) are provided as a Source data file.