Fig. 4: RNA-seq analysis reveals an EndoMT gene expression profile.
From: Slug regulates the Dll4-Notch-VEGFR2 axis to control endothelial cell activation and angiogenesis
a Left: Heat map for differentially expressed genes in GFP control and SlugOE EC on day 2 and day 6 of the fibrin-gel bead assay, grouped into 10 unique K-mean clusters. Right: Representative genes from each of the four main GSEA terms as shown in their respective K-mean clusters. b Differences in enrichment of indicated GO terms across 10 K-mean clusters. Length of the horizontal line for each cluster indicates the proportion of genes associated with that GO term that are enriched or reduced in the cluster. When present, + and – indicates the direction (positive and negative, respectively) of regulation for each biological process (Proliferation and Migration). Direction of regulation is not specified for Adhesion/Junction/ECM and Cytoskeleton/Shape. c Top two ranked Gene Ontology terms enriched in each K-mean group. d GSEA analysis showing enrichment in EMT-related terms in the gene set upregulated in SlugOE EC and downregulated in GFP control EC. e Immunofluorescence staining showing reduced surface expression of Claudin5 and VE-cad, but not CD31 in SlugOE EC. Scale bar: 50 µm. f 70 kDa rhodamine dextran perfusion of the vascularized micro-organ model at day 5 showing that network formed by SlugOE EC is leaky. Scale bar: 200 µm.
