Fig. 3: XBP1s regulates the hepatic 12-h cistrome.
From: XBP1 links the 12-hour clock to NAFLD and regulation of membrane fluidity and lipid homeostasis
a Binding profiles and heatmaps of XBP1s at the transcription start sites (TSSs) of 441 concordant 12-h oscillating genes in Xbp1flx/flx at CT0 (left) and CT8 (middle), and in the AlbCre;Xbp1flx/flx mice (right). The y axis represents the average signal in a 50-bp bin (normalized uniquely mapped reads). b Venn diagram of the 12-h transcriptome (n = 1886) and the 12-h XBP1s cistrome (n = 3649). Intersection: concordant 12-h oscillating genes (n = 441). c Top-enriched KEGG pathways identified in the concordant 12-h oscillating genes. d Top-enriched transcriptional factor motifs identified from concordant 12-h oscillating genes. e The proportion of XBP1s cistrome in the concordant 12-h oscillating genes. f 12-h rhythmic XBP1s cistrome binding detected by ChIP-Seq were plotted as binding profiles and heatmaps for the concordant 12-h oscillating genes every 4 CT hours for 36 h in total. g Representative views of 12-h rhythmic XBP1s ChIP-Seq data at the Ufl1, Txndc11, Mydgf, and Sec24c gene loci. h Top-enriched disease perturbations identified from concordant 12-h oscillating genes.
