Fig. 1: Identification of 13 transcriptionally distinct cell types in schistosomula. | Nature Communications

Fig. 1: Identification of 13 transcriptionally distinct cell types in schistosomula.

From: Single-cell atlas of the first intra-mammalian developmental stage of the human parasite Schistosoma mansoni

Fig. 1

a Experimental scheme describing the sources of the parasite material, single-cell analysis and validation pipeline. Approximately 5000 schistosomula per experiment were dissociated, followed by enrichment of fluorescein diacetate (FDA+) live cells using fluorescence-activated cell sorting (FACS). Cells were loaded according to the 10X Chromium single-cell 3’ protocol. Clustering was carried out to identify distinct populations and population-specific markers. Validation of population-specific markers was performed by in situ hybridisation (ISH). b Uniform Manifold Approximation and Projection (UMAP) representation of 3226 schistosomulum single cells. Cell clusters  are coloured and distinctively labelled. c Gene-expression profiles of population markers identified for each of the cell clusters. The colours represent the level of expression from dark red (high expression) to light red (low expression). The sizes of the circles represent the percentages of cells in those clusters that expressed a specific gene. The colour bars under gene IDs represent the clusters in (b).

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