Fig. 5: Target genes of Mll4 in developing mouse GHRH neurons.

a List of genes in developing mouse GHRH-neurons, which are associated with either Mll4 or Nrf1 ChIP-seq peaks or both Mll4 and Nrf1 ChIP-seq peaks. Genes encoding transcription factors are highlighted in blue. b Mll4 and Nrf1 ChIP-seq peaks for representative target genes of Mll4 in developing GHRH-neurons. The ___location of each peak with regard to its associated gene is marked by an arrow for the direction of the gene and a square for the 5′ UTR (both in blue). c Independent validation ChIP experiments with E15 hypothalamus and IgG (controls) and antibodies against Mll4 and Nrf1 reveal recruitment of Mll4 and Nrf1 to representative target genes of Mll4. Column bars represent mean of experiments with three independent embryos, error bars indicate the SD. d Luciferase reporter assays reveal that the common ChIP-seq peak areas for Mll4 and Nrf1 in Vat1 and Flywch2 are responsive to ectopic expression of Nrf1 and shRNA against Mll4. Column bars represent mean of three independent experiments, error bars indicate the SEM. Statistical differences were determined by two-sided Student’s t-test; not significant (ns), *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.