Fig. 6: Mll4-cKO show reduced expression of Mll4-target genes in developing GHRH-neurons. | Nature Communications

Fig. 6: Mll4-cKO show reduced expression of Mll4-target genes in developing GHRH-neurons.

From: The histone H3-lysine 4-methyltransferase Mll4 regulates the development of growth hormone-releasing hormone-producing neurons in the mouse hypothalamus

Fig. 6

a ISH analyses of expression of Mll4-target genes in Mll4-cKO and their littermate controls at indicated stages. b IHC analyses of coexpression of transcription factors whose genes are targeted by Mll4; the number of Dlx1 only, Prox1 only, and Dlx1/Prox1 double-positive cells at E17.5, and the number of Dlx1 only, Egr1 only, and Dlx1/Egr1 double-positive cells at P0 in Mll4-cKO and controls. The number of mice used is as indicated below each genotype in parenthesis (a, b). The ___location of ISH/IHC images is schematically shown (a), which applies to all images in (a, b). Statistical differences were determined by two-sided Student’s t-test; *p < 0.05, **p < 0.01, and ***p < 0.001. Column bars represent mean, error bars indicate the SD. Scale bars, 100 µm.

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