Fig. 5: Anticoagulant effects during extracorporeal circulation of human blood.

a Schematic drawing of the continuous recirculation within an ex vivo circuit that included a dialysis column and a reservoir (Blood pool). Whole blood from healthy donors is pumped via mechanical roller pump to the dialyzer through the closed extracorporeal circuit, while the dialysate solution flows in the opposite direction through the device and into a waste container (Dialysate drain). The dialyzer and dialysate are heated to ~30 °C. b The plasma clotting time (APTT) in the presence of one of the anticoagulant strategies (Ori + T + H or Aptarray) at the indicated time points. The samples were collected from the infusion port. c The representative photographs (scale bars, 2 cm) and SEM images (scale bars, 200 μm) of filters collected from the post circulation dialyzer after following the different anticoagulation strategies.