Fig. 4: Metabolic effects of MT1LKO mice.
a MT1-MMP deletion. Whole-cell lysate of primary mouse hepatocytes isolated from MT1Flox and MT1LKO mice was subjected to immunoblotting. Similar results were obtained from three independent experiments. b, c Relative expression of target genes. qRT-PCR measurement of mRNA levels of MT1-MMP in different tissues (b) (four or five mice per group) or MT1-MMP, MT2-MMP, and Adam17 in the liver (five or six mice per group) (c). The relative mRNA levels were the ratio of the mRNA levels of the target genes from different tissues to that of Gapdh in the same tissue. WAT, white adipose tissue. d Liver LDLR levels (6 mice per group). Liver homogenate was subjected to immunoblotting. Relative densitometry was the ratio of the densitometry of LDLR of different mice to that of actin of the same mouse. Representative images were shown. Similar results were obtained from the other three mice. e Plasma sLDLR determined by ELISA (6 mice per group). f Relative mRNA levels (4 mice per group). g Plasma levels of total cholesterol (6 mice per group). h Lipid profile. Same amount of plasma from each mouse in the same group was pooled and applied to FPLC analysis of plasma cholesterol (6 mice per group). i–k Expression of human MT1-MMP. MT1Flox and MT1LKO mice were injected with AAVs encoding GFP or human MT1-MMP, respectively. Liver homogenate was applied to immunoblotting (6 mice per group). Representative images were shown. Similar results were obtained from the other three mice (i). Plasma samples were used to measure sLDLR levels (6 mice in the control group and 5 mice in MT1-MMP overexpression group) (j), and total cholesterol levels (6 mice per group) (k). Student’s t test (two-sided) was carried out to determine the significant differences between groups (b–g, j and k). The significance was defined as p < 0.05. Values of all data were mean ± SD. Source data are provided as a Source Data file.
