Fig. 2: Arrest of Rax+ tanycyte lineage during homeostasis.

a Representative images showing that tdTomato⁺ cells from Rax-CreER^T2::Ai14 mice are positive for tanycyte marker Scn7a at 1 dpi with tamoxifen. The Scn7a mRNA was detected by smFISH. Scale bar: 50 µm. b Lineage tracing of Rax⁺ tanycytes in ME at the population level using Rax-CreER^T2::Ai14 mouse line. Shown are the confocal images of tdTomato⁺ cells in ME at 3, 7, 14, and 30 dpi with tamoxifen. Scale bar: 50 µm. c Quantification of the percentage of tdTomato⁺ cells distributed in EZ and SEZ. Values are presented as mean ± SEM, and significance was analyzed by one-way ANOVA with Sidak’s multiple comparison test (n = 3 biologically independent animals). N.S. not significant versus 3 dpi. d Representative confocal images obtained from fate mapping of Rax⁺ tanycytes show the costaining of tdTomato with different cell markers Sox2, NG2, NeuN, CC1, and S100β. White arrowheads indicate marker-positive cells and purple arrowheads signify tdTomato⁺ cells. Scale bar: 20 µm. e Quantification of the percentage of tdTomato⁺ cells expressing different cell markers. Values represent mean ± SEM (n = 3, 4, 3, and 3 mice for 3, 7, 14, and 30 dpi groups). f Shown are representative confocal images stained for Sox2, tdTomato, and EdU in ME of Rax-CreER^T2::Ai14 mice. Scale bar: 20 µm. g Percentage of EdU⁺ mitotic cells among tanycytes. Source data are provided as a Source Data file. The precise P values are summarized in Supplementary Data 3.