Fig. 4: Analysis of the state and number of SCs 14 and 30 days after CTX injection.

a, g Schematic representation of the experimental design. b, h Immunostaining of Pax7 and MyoD in TA muscle sections. Scale bars = 50 µm. Representative regions are shown at higher magnification. Scale bars = 25 µm. White arrows indicate Pax7⁺MyoD⁺ myoblasts and Red arrows indicate Pax7⁺MyoD⁻ SCs. c, i Quantification of Pax7 and the percentage of Pax7⁺MyoD⁻ SCs in TA muscle sections. d, j Immunostaining of embryonic myosin heavy chain (eMHC) and Dystrophin in TA muscle sections, and the quantification of the percentage of immature myofibers that express eMHC. Scale bars = 50 µm. Similar results were repeated independently in four pairs of mice. In addition, similar results were repeated three times for each mouse. e, k Mallory’s trichrome stain of TA muscle sections. Scale bars = 100 µm. Similar results were repeated independently in four pairs of mice. In addition, similar results were repeated three times for each mouse. f, l H&E staining of TA muscle sections and myofiber size distributions in TA muscle sections. Scale bars = 100 µm. a–f represents the analysis of SCs 14 days after CTX injection, and g–l represent the analysis of SCs 30 days after CTX injection. Error bars represent mean + SD of four mice. A two-sided unpaired Student’s t-test was performed.