Fig. 4: Wilms tumor and clear cell sarcoma of the kidney.
From: Single cell derived mRNA signals across human kidney tumors
A Bulk Wilms tumor and CCSK compared to fetal kidney: The relative contribution of single cell derived signals from fetal kidney in explaining the bulk transcriptomes of 137 nephroblastomas (Wilms tumors) and 13 CCSKs along with control populations. The relative contribution of each signal to each bulk RNA-seq sample is shown by the y-axis. Each signal/sample combination is represented by a single point and boxplots shows the distribution with median (middle line), 1st and 3rd quartiles (box limits) and 1.5 times the inter-quartile range (whiskers). Each signal type is abbreviated and colored as per the legend, with squares for fetal and circles for mature. Wilms/CCSK samples are shown on the left and control samples on the right, where “Leukocytes” are bulk transcriptomes from flow sorted leukocytes and “Leukemia” represent B-precursor acute lymphoblastic leukemia. B UMAP of CCSK and Wilms single cell transcriptomes: Points represents cell transcriptomes from Wilms tumor (left) or CCSK (right), with shading, contours, and labels indicate cell type. C Comparison of CCSK and Wilms transcriptomes to reference signals: Similarity of transcriptomes from B (rows) to fetal kidney reference signals (columns), where color indicates logit similarity. D Comparison of unexplained signal contribution to CCSKs and other tumor types: For each group of samples, the unexplained signal is calculated using the reference set of signals given at the top (e.g., fetal kidney). The unexplained signal fractions are shown by black bars, sorted in increasing order, with the red horizontal line showing the median value and the vertical line the range between the 25th and 75th percentiles. CCSK samples were fitted using 5 different reference sets (fetal and mature kidney, mature kidney only, fetal adrenal, mouse embryo, and the pan-tissue human cell landscape). The final group on the right, represents samples fitted using inappropriate references. This population serves as a calibration of the expected level of unexplained signal when the bulk transcriptome is not explained by any of the provided reference signals. Source data are available as a Source Data file.
