Fig. 8: Cpd G reduced accumulation of cholesterol esters in renal cortex in both disease models.

a–c Lipid accumulation in kidneys from mice that received vehicle (V, n = 8) or Cpd G (n = 10) for 28 days after ADR challenge. A group that did not receive ADR (C) (n = 10), is included to show the healthy phenotype. a Representative image of Oil-red O (ORO) stained kidney sections; b cholesterol ester (CE) content, normalized to total proteins in kidneys. Data expressed as the median and range. Differences with regard to animals injected with ADR that received vehicle were calculated using Kruskal–Wallis, followed by Dunn’s test: no ADR, P < 0.001; ADR+ Cpd G, P = 0.003; c cholesterol ester content in kidneys from individual animals vs the corresponding albuminuria (ACR) values at day 28 of treatment. The data points in red correspond to the group that received Cpd G. A two-tailed Spearman correlation coefficient test was used to assess the relationship between the variables (n = 17 pairs, r = 0.838, P < 0.0001). d Cholesterol ester content (CE), normalized to total proteins, in the kidney cortexes from 8-week-old Col4a3 KO mice, that received vehicle or Cpd G for 28 days. A group of Col4a3+/+ littermates of the same age (C), is included to show the healthy phenotype. Data expressed as the median and range. Col4a3 KO mice treated with vehicle or with Cpd G were compared using a double-tailed Mann–Whitney test (n = 4, U = 0, P = 0.0286).