Fig. 7: WFS1 recognizes vesicular cargo proteins via its luminal C-terminal segment.
From: WFS1 functions in ER export of vesicular cargo proteins in pancreatic β-cells
a The interaction between different mutant WFS1 proteins and vesicular cargo proteins evaluated by BiFC and then quantified by flow cytometry. n = 5 independent experiments. b Representative imaging of the interaction between different mutant WFS1 and vesicular cargo protein SCG5 by PLA. The puncta number per cell was quantified by ImageJ software. n = 4 independent experiments, n = 8 independent images quantified. Scale bar, 10 μm. c A model of WFS1 mediating vesicular cargo protein transport from the ER. All the data are presented as mean ± s.e.m. p < 0.05, significant, using a two-tailed Student’s t-test. Source data are provided as a Source Data file.
