Fig. 1: Chronic inhibition of the mouse PFC results in rsfMRI overconnectivity.
From: Increased fMRI connectivity upon chemogenetic inhibition of the mouse prefrontal cortex
a Viral expression localization. The potassium channel Kir2.1 (n = 16) or GFP (control, n = 19) were transduced bilaterally into the PFC of adult male mice. Left: representative histology sample showed Kir2.1 (green). Right: heatmaps illustrate a qualitative regional assessment of viral expression across subjects. b Seed-based connectivity mapping of the PFC in GFP (control), and Kir2.1-transduced subjects. c Corresponding group difference maps. Area exhibiting significantly increased rsfMRI connectivity in Kir2.1 expressing mice are depicted in red-yellow (r and T stat difference map). d Antero-posterior profiling of rsfMRI connectivity of the PFC within the midline axis of the mouse DMN (§p = 0.014, two-way ANOVA with repeated measurements, genotype effect, n = 16 and n = 19 Kir2.1 or GFP-expressing mice, respectively). e Fronto-thalamic rsfMRI overconnectivity in Kir2.1 expressing mice (*p = 0.014, two-sided t test, n = 16 and n = 19 Kir2.1 or GFP-expressing mice, respectively). Data in e and f are presented as mean values ± SEM. f Regions exhibiting rsfMRI overconnectivity in Kir2.1 mice are robustly innervated by the PFC. Left: axonal projections from the PFC (top 20% strongest connections). Middle: scatter plot illustrating intergroup differences in rsfMRI connectivity as a function of PFC structural connectivity strength. Green dots indicate significantly functionally overconnected voxels. Right: Distribution of overconnected voxels as a function of axonal connectivity strength . FC functional connectivity, DMN Default Mode Network, Cg cingulate cortex, PFC prefrontal cortex, Rs retrosplenial, Th thalamus. Source data are provided as a Source Data file.
