Fig. 7: ADARs-regulated circRNAs affect tumorigenesis.
From: ADARs act as potent regulators of circular transcriptome in cancer
a Fold change in expression of circRNAs (circular) and their corresponding host genes (linear) in EC109 (upper panel) and SNU398 cells (lower panel), upon CasRX and guide RNA-mediated knockdown of circRNAs (gCirc) compared to non-targeting control (gNT). gNT-circular, circRNA expression of indicated gene upon treatment of CasRX and non-targeting guide RNA, and so forth. b, c Quantification of foci (b; scale bar, 1 cm) and colonies formed in soft agar (c; scale bar, 200 µm) by EC109 cells with or without circRNAs knockdown. d, e Quantification of foci (d; scale bar, 1 cm) and colonies formed in soft agar (e; scale bar, 200 µm) by SNU398 cells with or without circRNAs knockdown. f, g Images and growth curves of xenograft tumors derived from EC109 (n = 6) (f) and SNU398 cells (n = 5) g. Data are presented as the mean ± S.D. of tumor volumes. Statistic significance was calculated by unpaired, two-tailed Student’s t-test; *, P < 0.05; **, P < 0.01. a–e Each dot represents the mean value of technical triplicates. Data are presented as the mean ± S.D. of 3 biological replicates. Statistical significance was calculated by paired, two-tailed Student’s t-test; n.s., not significant; *, P < 0.05; **, P < 0.01; ***, P < 0.001. Exact P values and source data are provided in Source Data file.
