Fig. 4: Loss of Sirt6 partially normalizes aberrant expression patterns in dystrophic mdx muscles.
a Volcano plot of differentially expressed genes in mdx compared to control muscles. Benjamini-Hochberg test, two-sided. b Volcano plot of differentially expressed genes in mdx compared to Sirt6mKO/mdx muscles. Benjamini-Hochberg test, two-sided. c Venn diagram of overlapping upregulated genes in mdx muscle compared to control muscle and downregulated genes in Sirt6mKO/mdx muscle compared to mdx muscles based on RNA-seq. In total, 257 overlapping genes were categorized by Wikipathways analysis. Fisher’s exact test, two-sided. d Venn diagram of overlapping downregulated genes in mdx compared to control muscle and upregulated genes in Sirt6mKO/mdx muscle compared to mdx muscle based on RNA-seq. In total, 266 overlapping genes were categorized by Wikipathways analysis. Fisher’s exact test, two-sided. e Heat map of expression of genes involved in cellular stress responses in control, mdx and Sirt6mKO/mdx muscle (n = 3 for each group). f RT-qPCR analysis of expression of indicated genes involved in cellular stress responses in control, mdx and Sirt6mKO/mdx muscles. m36b4 was used as reference gene (one-way ANOVA with Bonferroni multiple comparisons test: Dab2: *p = 0.0235, **p = 0.0017; ****p < 0.0001; Hspa2: ns not significant, *p = 0.0164, **p = 0.0012; Ptpn22: **p = 0.009, *p = 0.0316, *p = 0.0430; n = 4 for each group). Twelve to 16 weeks old male mice were used. Data are presented as mean ± SEM. Source data are provided in the Source Data file.
