Fig. 2: Single-particle cryo-EM structures of mSMO-PGS.
From: Fusion protein strategies for cryo-EM study of G protein-coupled receptors
a The atomic models of mSMO-PGS1 (blue) and mSMO-PGS2 (yellow) predicted by alphaFold2 (AF2). Note that the inserted PGS are predicted to be oriented in two opposite directions in these two constructs. b Cryo-EM density determined from mSMO-PGS1, docked with the atomic model predicted by AF2 (blue ribbon). c Cryo-EM density determined from mSMO-PGS2, docked with the atomic model predicted by AF2 (yellow ribbon). d Overlay of determined structure (pink ribbon) and predicted atomic model for mSMO-PGS2. e–g Enlarged views of connection region between SMO TM6 and PGS in the predicted model of mSMO-PGS1 (e), predicted model of mSMO-PGS2 (f), and the determined structure of mSMO-PGS2 (g).
