Fig. 6: Upregulation of PIM1 in VKH patients.
From: Multicellular immune dynamics implicate PIM1 as a potential therapeutic target for uveitis
a t-SNE plots of T cell subsets from all healthy controls and VKH samples. b t-SNE plots of B cells subsets from all healthy controls and VKH samples. c Violin plots of PIM1 expression in T cell subsets of healthy controls and VKH patients. d Violin plots of PIM1 expression in B cell subsets of healthy controls and VKH patients. e Heatmap of the average expression of PIM1 by PCs and CD4 T cell subsets in healthy controls and VKH patients. f, g The percentage of PIM1+ cells in CD4 T cells (f) and PCs (g) measured by flow cytometry. Each group contains 15 samples. P(CD4 T cells, VKH-HC) = 0.0010, P(PCs, VKH-HC) = 0.0011. Data expressed as mean ± SEM. Significance was determined using unpaired two-tailed student’s t test. **P < 0.01, ***P < 0.001. h, i The proliferating rate of CD4 T cells (gated on CD3+CD8- T cells) (h) and B cells (i) measured by flow cytometry. P(CD4 T cells, SMI-4a (−)-SMI-4a (+)) = 6.4E-08, P(PCs, SMI-4a (−)-SMI-4a (+)) = 7.1E-08. Data represented as mean ± SEM from six independent experiments. Significance was determined using unpaired two-tailed student’s t test. ****P < 0.0001.
