Fig. 7: Targeted activation of CapChR2 reliably elicits calcium influx to single Drosophila neurons.
From: Calcium-permeable channelrhodopsins for the photocontrol of calcium signalling
A Expression of UAS-CapChR1, UAS-CapChR2 and UAS-CrChR2 was targeted to M4/6 mushroom body output neurons using the VT1211-Gal4 driver line. B Blue-light (470 nm, ~4.5 mW/cm2) activation of CapChR2 elicits marked calcium increase in M4/6 neurons, measured with co-expressed jRCaMP1a in Drosophila explant brain preparations. C Example jRCaMP1a fluorescence traces following blue-light pulses of indicated duration (blue bars). D Fluorescence signal quantification following blue-light stimulation. For 0.05 s, 0.5 and 1 s of blue light excitation n = 16 for CapChR1, n = 13 for CapChR2, n = 10 for CrChR2 (C2) and n = 8 for the control. For 2 s blue light excitation n = 12 for CapChR1, n = 7 for CrChR2 and n = 6 for the control. Bar charts depict the mean ± SEM. n = X biologically independent flies. AU arbitrary units.
