Fig. 2: Long-term statins administration worsens renal injury in db/db mice. | Nature Communications

Fig. 2: Long-term statins administration worsens renal injury in db/db mice.

From: Long-term statins administration exacerbates diabetic nephropathy via ectopic fat deposition in diabetic mice

Fig. 2

All mice were ~50 weeks old. a Representative images of mice kidney. b Representative sections of PAS staining for glomerulus. Arrows represent mesangial expansion and thickened capillary loop. Original magnification ×1000, Scale bar: 20 µm. c Representative images of TEM. The ultrastructure of renal cortex under electron microscope was shown in the enlarged pictures, and the stars in pictures were used to marked the basement membrane thickening and foot process fusion. Original magnification ×9700 and ×26,500, scale bar: 1 and 0.5 µm. d Representative sections of Periodic Acid-Silver Methenamine (PASM) staining for glomerulus. Arrows represent histologic abnormalities in the glomerulus, such as the increase of mesangium and GBM expansion, in the statin administration groups. Original magnification ×1000, Scale bar: 20 µm. e Immunofluorescence of Wilm tumor gene1 (WT1) staining. FITC-labeled WT1 (green) and DAPI (nuclei, blue) were used. WT1 is mainly expressed on the nucleus of glomerular podocytes, and the specific positive staining is indicated by the arrows48. Original magnification ×1000, Scale bar: 10 µm. f Immunofluorescence of Nephrin staining. Alexa Fluor 594-labeled Nephrin were used. Nephrin is mainly expressed on the plasma membrane domains, and arrows represent Nephrin positive staining49. Original magnification ×1000, Scale bar: 10 µm. g Glomerular Volume (103 × μm3). h Quantification of the mesangial area glomerulus. i Quantification of the number of podocytes in glomerulus. n = 6 in each group. j Immunofluorescence analysis of Nephrin. k Creatinine. l GFR measurement. n = 4 in Db/m and Db/db group, n = 6 in Db + Ato10 group. m UACR (n = 4 per time point). Data are expressed as means ± SEM. Significance **p < 0.01 versus Db/m group; ***p < 0.001 versus Db/m group. n Urinary KIM1 levels (n = 4 per time point). Significance #p < 0.01 versus Db group, ###p < 0.001 versus Db group. n = 6 in each group (g–k). Data are expressed as means ± SEM (g–l). One-way ANOVA with Tukey post hoc test was used for the analysis of statistical significance. Source data are provided as a Source Data file.

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