Fig. 3: Identification of FLC as the gene responsible for the loss of the vernalization requirement in pop3.
From: Common evolutionary trajectory of short life-cycle in Brassicaceae ruderal weeds
a, b Flowering phenotypes (a) and flowering time (b) of the Pudong and HANGYY8055 accessions and Pudong × HANGYY8055 F1 plants grown in LD in a growth chamber. +/−V, with or without vernalization treatment. Scale bar in (a) 5 cm. c Flowering time of the Pudong and HANGYY8055 accessions and plants in the Pudong × HANGYY8055 F2 segregating population in LD. d Genome-wide Δ(SNP index) plot from BSA of the F2 segregating population derived from a cross between Pudong and HANGYY8055. The black lines indicate tricube-smoothed Δ(SNP index), and gray lines represent the corresponding two-sided 99% confidence intervals. e The Δ(SNP index) plot of Chr 6D from BSA of the F2 segregating population derived from a cross between Pudong and HANGYY8055. The black line indicates tricube-smoothed Δ(SNP index), and gray lines mark the corresponding two-sided 99% confidence intervals. The blue dotted line indicates the ___location of C. occulta FLC (CoFLC) on Chr 6D. f The gene structure of CoFLC and the ___location of the mutation (blue line). Black boxes, gray boxes, and black lines represent exons, untranslated regions (UTRs), and introns, respectively. g Analysis of the transcript levels of CoFLC genes on Chr 6D in the Pudong and HANGYY8055 accessions by RNA-seq. Normalized counts and adjusted P values were both analyzed by DESeq2. The P values attained by the two-sided Wald test were corrected for multiple testing using the Benjamini and Hochberg methods. Data are mean ± s.e. of three biological replicates (open circle). ns, not significant. h Flowering time of Arabidopsis FRISF2 FLC, FRISF2 flc, and T1 transgenic lines in LD. We generated an allele of Arabidopsis FLC (FLCL160*) that mimics the mutation in FLC allele of pop3. Plants were grown in a growth chamber. Letters indicate significant differences as determined by ordinary one-way ANOVA. Error bars in b denote s.d. The number of examined plants (n) is given. The centers of the error bars represent the mean values. The source data underlying Fig. 3b, c, and h are provided as a Source Data file.
