Fig. 5: Effects of the expression of different alleles of TgAMPKα on the intracellular replication (a), invasion efficiency (b), ATP level (c), and nascent protein synthesis activity (d) of TgAMPKγ depleted parasites.

All experiments were repeated n = 3 (a–c) or n = 4 (d) times independently and the Means ± SEM are graphed. ***P = 0.0004, ****P < 0.0001, two-way ANOVA with Tukey’s multiple comparisons post-tests (a), unpaired two-tailed Student’s t-test (b–d). The replication and invasion assays were performed as in Fig. 2d and f. The ATP level was measured by a commercial luciferase assay using lysates prepared from 5 × 10⁶ mechanically released fresh parasites that were treated with or without IAA for 48 h. The nascent protein synthesis activity was determined by adding L-homopropargylglycine, an alkyne-tagged analog of methionine, to parasites that were treated with or without IAA. Then the incorporated L-homopropargylglycine was detected by a click chemistry approach using the fluorescent probe FAM azide and quantified by flow cytometry analyses. Source data are provided as a Source data file.