Fig. 6: Effects of the AMPK activator A769662 and inhibitor compound C (Comp C) on parasite replication and invasion. | Nature Communications

Fig. 6: Effects of the AMPK activator A769662 and inhibitor compound C (Comp C) on parasite replication and invasion.

From: Rapid metabolic reprogramming mediated by the AMP-activated protein kinase during the lytic cycle of Toxoplasma gondii

Fig. 6

a intracellular replication assays of RH in the presence of indicated treatments, as done in Fig. 2d. Invaded parasites were allowed to replicate for 24 h in MEF cells lacking both AMPKα1 and AMPKα2. Means ± SEM of n = 3 independent experiments, ****P < 0.0001, *P = 0.0201, two-way ANOVA with Tukey’s multiple comparisons post-tests. b efficiency of host cell invasion determined by a two-color assay that discriminated invaded vs non-invaded parasites. The RH parasites were treated with indicated chemicals for 1 h before egress. Then they were released by needle passage and purified parasites were used to infect HFF cells for 10 min in the presence of corresponding compounds. Subsequently the number of invaded parasites were determined by IFA. Invasion efficiency was plotted as the number of invaded parasites divided by the number of host cells. Means ± SD of n = 3 independent experiments, *P = 0.0103, **P = 0.0015, unpaired two-tailed Student’s t-test. Source data are provided as a Source data file.

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