Fig. 1: Restricted functional antitumor activity of T-αFGL2 cells in vitro. | Nature Communications

Fig. 1: Restricted functional antitumor activity of T-αFGL2 cells in vitro.

From: FGL2-targeting T cells exhibit antitumor effects on glioblastoma and recruit tumor-specific brain-resident memory T cells

Fig. 1

a Schematic of the vector encoding the FGL2-blocking scFv. b Schematic of a T cell transfected with the FGL2-blocking scFv vector (T-αFGL2). c Representative flow cytometry histograms demonstrating expression of the FGL2-blocking scFv on mouse T cells following transduction. d Flow cytometry plots depicting no difference in proportion of tumor cells (DBT-GFP+) cocultured with T-Ctr or T-αFGL2 cells at E:T ratio of 4:1 for 72 h (top panel); flow cytometry plots depicting higher granzyme B expression and no difference in TNFα or IFNγ expression in T-αFGL2 cells (compared with T-Ctr) cocultured with DBT tumor cells at E:T ratio of 1:1 for 24 h. Data are mean ± SD from three independent experiments. NS not significant, two-tailed t-test. e Representative micrographs of FGL2 expression in GBM and the indicated normal human tissue samples. The images of normal human tissue shown are representative results from two samples. Micrographs are representative of two sections per tissue sample. Scale bars, 100 μm.

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