Fig. 4: Loss of SAPS3 reverses metabolic and transcriptional alterations induced by HFD. | Nature Communications

Fig. 4: Loss of SAPS3 reverses metabolic and transcriptional alterations induced by HFD.

From: SAPS3 subunit of protein phosphatase 6 is an AMPK inhibitor and controls metabolic homeostasis upon dietary challenge in male mice

Fig. 4

a Heatmap of significantly changed metabolites in liver samples from FF and LKO mice fed with different diets was analyzed by LC-MS. n = 3 mice per group analyzed by one-way ANOVA. b Pathway analysis of upregulated metabolites and downregulated metabolites from FF and LKO mice respectively. n = 3 mice per group analyzed by unpaired t-test. c Transcriptional profile heatmap denoting significantly changed genes in liver samples from FF and LKO mice fed with different diets. CD n = 4 mice per group; HFD, n = 5 per group. Fold change > 1.5, p < 0.05 and FDR < 0.05. d Distribution in genes that were changed in FF mice by HFD and completely reversed (78%), partially reversed (3%), no difference (18%), or augmented (1%) by the knockout of SAPS3. e KEGG pathway analysis of the significantly reversed genes by SAPS3 knockout. CD n = 4 mice per group; HFD, n = 5 per group. Fold change > 1.5, p < 0.05, and FDR < 0.05. f, g GSEA analysis of the genes from FF and LKO mice under HFD. Adipogenesis and long-chain fatty acid synthesis were identified and shown in g.

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