Fig. 1: LpWF stably colonizes the fly gut with spatial specificity. | Nature Communications

Fig. 1: LpWF stably colonizes the fly gut with spatial specificity.

From: A symbiotic physical niche in Drosophila melanogaster regulates stable association of a multi-species gut microbiota

Fig. 1

A Colonization assay schematic with initial dosing on day 0 and serial transfers to sterile food daily for 3 days before analysis. B Gut diagram. C Microscopy of LpWF-mCherry colonization in a whole gut after clearing transient cells shows a specific colonization zone in the foregut. Shown is a maximum-intensity z-projection. D The proventriculus is a major site of LpWF colonization. E Ai colonization is also specific to the proventriculus lumen and crop duct (see also Fig. S2). F CFU densities from regions dissected in B. n = 23 individual guts/region from three biological replicates. Columns represent means. Error bars are S.D. G Microsurgery was carried out to remove the crop. H LpWF colonizes the foregut of flies with the crop removed (n = 15/15). I TEM cross-section of proventriculus inner lumen. Representative image of n = 3 biological replicates. J Detail of (I). Scale bars are defined in the figure panels. Source data are provided as a Source Data file.

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