Fig. 2: Kinetic properties of bacterial association suggest the existence of a niche in the proventriculus. | Nature Communications

Fig. 2: Kinetic properties of bacterial association suggest the existence of a niche in the proventriculus.

From: A symbiotic physical niche in Drosophila melanogaster regulates stable association of a multi-species gut microbiota

Fig. 2

A Saturation occurs over a colonization time course of germ-free flies by LpWF. Data points are mean of log10(CFUs) in n ≥ 48 flies/data point. Error bars represent 1 s.e.m. Inset: 20-day time course after inoculation with 106 CFUs (data from24). B Bacterial pulse-chase experimental design: flies were first pre-colonized with LpWF-mCherry, then fed an excess of unlabeled LpWF (blue) daily on fresh food. C Acterial cell turnover quantified by pulse-chase time course of Lp-mCherry-pre-colonized flies continuously fed unlabeled LpWF or Ai-GFP-pre-colonized flies continuously fed unlabeled Ai. Data points are mean of log10(CFUs) in n ≥ 34 flies/data point. Error bars represent 1 s.e.m. D Colonization efficiency quantified by dose response to colonization of individual flies. CFUs were measured at 3 dpi of the second colonizer. n = 24 flies/dose, error bars represent 1 standard error of the proportion. Limit of detection: 50 CFUs. E Spatial structure of colonization dynamics in the proventriculus for a fly pre-colonized with LpWF-mCherry (red) invaded by LpWF-GFP and imaged 1 h post inoculation (hpi). F Optical x,z-slice. Source data are provided as a Source Data file.

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