Fig. 4: Colonization of the niche induces morphological alteration of the proventriculus. | Nature Communications

Fig. 4: Colonization of the niche induces morphological alteration of the proventriculus.

From: A symbiotic physical niche in Drosophila melanogaster regulates stable association of a multi-species gut microbiota

Fig. 4

A Xray µCT model of a whole fly. Cutaway shows (1) exposed proventriculus (also inset of (B)), (2) anterior midgut, and (3) posterior midgut. B Detail of proventriculus. C Cross-section of a germ-free proventriculus inner lumen. Scale bar: 5 µm. D Germ-free proventriculus inner lumen volume rendering. E LpWF-colonized proventriculus inner lumen cross-section. Scale bar: 5 µm. F LpWF proventriculus inner lumen volume rendering. G Cardia volume calculated from surface models (n = 3 to 4 flies per condition; p = 0.0025, one-way ANOVA relative to germ-free; Tukey’s correction for multiple comparisons; GF vs. Lp p = 0.020; GF vs. Lp+Ai p = 0.022.). H–M Transmission electron microscopy transverse cross-section of anterior proventriculus in (H) germ-free fly, (I) conventionally-reared fly (only lab fly bacteria; no LpWF), ( J, K) 1 hpi with LpWF, (L, M) 3 dpi colonized with LpWF (see Fig. S7). n ≥ 3 biological replicates per treatment for TEM. Yellow arrowheads indicate lumen space. Source data are provided as a Source Data file.

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