Fig. 3: The risk allele of rs2238574 enhances the chromatin binding of transcription factors. | Nature Communications

Fig. 3: The risk allele of rs2238574 enhances the chromatin binding of transcription factors.

From: A regulatory variant at 19p13.3 is associated with primary biliary cholangitis risk and ARID3A expression

Fig. 3

A, B ChIP-qPCR confirmation of the enrichment of active enhancer marks at rs2238574-containing region in K562 cells and human CD14+ monocytes. C Motif analysis suggests that rs2238574 resides within DNA-binding motifs of PPARG, NR2F1 and NR2C2. D ChIP-qPCR results showing enrichment of PPARG, NR2F1 and NR2C2 at the rs2238574-containing region in K562 cells. E ChIP-AS-qPCR showing allele-specific binding of PPARG, NR2F1 and NR2C2 at rs2238574 in K562 cells. F ChIP-qPCR results showing enrichment of PPARG, NR2C2 and NR2F1 at the rs2238574-containing region in human CD14+ monocytes. G ChIP-AS-qPCR showing allele-specific binding of PPARG, NR2C2 and NR2F1 at rs2238574 in human CD14+ monocytes. H Sanger sequencing chromatograms of the SNP region from the input and ChIPed DNA in human CD14+ monocytes. Data are representative of three independent experiments (Mean ± SD for triplicates). P values are calculated using unpaired two-tailed Student’s t test and indicated significant if *p < 0.05, **p < 0.01, or ***p < 0.001.

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