Fig. 2: L6-F4-2 treatment rescues retinopathy in Ndp^KO mice.

a–d WT (control) and Ndp^KO male and female mice were treated with PBS or L6-F4-2 by single intravitreal injection (0.19 μg) at P0 and retinas were harvested at P8. a Whole-mount isolectin GS-IB4 labeling of retinal veins. Representative images of the whole retinal vascular plexus, quadrant vascular plexus, angiogenic front and central plexus are shown. Scale bars represent 0.5 mm (top), 0.3 mm (middle) and 0.15 mm (bottom two rows). b Quantification of vessel diameter, vessel density and distance from the optic nerve from (a). c Whole-mount anti-smooth muscle actin labeling of retinal arteries at P8. d Quantification of artery branches from (c). Scale bar represents 0.5 mm. In all the quantifications, error bars represent mean ± SEM, Ndp WT n = 7 mice, Ndp^KO n = 8 mice, *p < 0.05, **p < 0.01, ***p < 0.001; two-sided Mann–Whitney U test. e WT (control) and Ndp^KO mice were treated with PBS or L6-F4-2 by intravitreal injection (0.19 μg) at P5 and every three days with retina harvest at P21. Optical sections of isolectin-stained P21 retinas showing the three-layered retinal vasculature (superficial, intermediate, and deep layers) to document vascular architecture. Scale bars represent 100 μm. f Vascular density quantification of retina intermediate and deep layers from (e), n = 3 mice, ***p < 0.001, ****p < 0.0001, one-way ANOVA. Source data are provided as a Source Data file. For box plots, whiskers indicate the minimum and maximum values in the dataset. The center is the median number. Boundaries of boxes are the first quartile and third quartile.