Fig. 5: Laptm5-HKO exacerbates HFHC-induced NASH.

a Fasting blood glucose of Laptm5-HKO and Laptm5-Flox mice for NC or HFHC consumptions (n = 10 mice/group). b and c Liver weight and LW/BW (b), and hepatic TG, TC contents (c) of Laptm5-HKO and Laptm5-Flox mice after NC or HFHC feeding for 8 or 16 weeks (n = 10 mice/group). d H&E (upper) and Oil Red O (lower) staining in the liver sections of mice in the indicated groups (n = 6 mice/group). Scale bar, 100 μm. e and f NAS score analysis (e) and the statistical analysis of Oil red O staining (f) of Laptm5-HKO and Laptm5-Flox mice after NC or HFHC feeding for 8 or 16 weeks (n = 6 mice/group). g Immunofluorescence staining (g) and statistical analysis (h, i) of CD11b (red) in the liver sections of mice in the indicated groups. (Nuclei, blue) (n = 4 mice/group). Scale bar, 50 μm. PSR staining of mice liver sections in the indicated groups. (8 weeks, n = 6 mice/group, 16 weeks, n = 7 Laptm5-Flox mice and n = 5 Laptm5-HKO mice). Scale bars, 100 μm. j Serum ALT and AST concentrations of mice in the indicated groups (n = 10 mice/group). k Hierarchical clustering analysis of the RNA-seq data from the mice fed the HFHC diet. l and m GSEA pathway enrichment analysis of pathways related to lipid metabolism, inflammation, apoptosis, and fibrosis. n Heatmaps of the genes related to lipid metabolism, inflammatory responses, and fibrosis (red, upregulated; blue, downregulated) in the indicated groups. Data are represented as mean ± SD. The Mann–Whitney U nonparametric statistical test was used for statistical analysis in (c—8w, e—16w, and i—16w) and two-tailed Student’s t-test in other panels. Source data are provided as a Source data file.