Table 1 Data collection and refinement statistics

PDB code

7WNT

7WNU

Space group

P6422

C222₁

Unit cell a,b,c (Å)

128.52 128.52 184.276

92.06 145.38 192.1

α, β, γ [°]

90 90 120

90 90 90

Resolution range (Å)^a

44.4–2.45 (2.53–2.45)

41.51–3.20 (3.31–3.20)

Total reflections

67687 (6538)

43318 (4314)

Unique reflections

33845 (3269)

21660 (2157)

Completeness (%)

99.65 (98.58)

99.80 (99.72)

I/σ(I)

22.93 (2.43)

16.77 (5.24)

Wilson B-factor

58.61

59.25

R-merge^b

0.016 (0.286)

0.040 (0.156)

R-meas

0.022 (0.404)

0.057 (0.221)

R-pim

0.016 (0.286)

0.040 (0.156)

CC1/2

1 (0.872)

0.998 (0.973)

Resolution (Å)

44.4–2.45 (2.53–2.45)

41.51–3.20 (3.31–3.20)

Reflections used in refinement

33789 (3267)

21630 (2151)

Reflections used for R-free

1628 (139)

1067 (93)

R_work^c

0.219 (0.327)

0.185 (0.255)

R_free^d

0.251 (0.369)

0.240 (0.356)

No. residues

529

1111

Bond lengths (Å)

0.009

0.011

Bond angles (Å)

1.33

1.34

Average B-factor

63.79

63.03

Ramachandran favored (%)

96.18

89.57

Ramachandran allowed (%)

3.82

10.43

Ramachandran outliers (%)

0

0

Protein or RNA

3882

8536

Ligands or ions

2

4

water

86

0

B-factors

Protein or RNA

63.99

63.03

Ligands or ions

60.73

66.61