Fig. 5: ITF2 binds to p65 and interrupts p65 translocation into the nucleus, resulting in reduced TNF-induced p65 activation.
From: Protein stabilization of ITF2 by NF-κB prevents colitis-associated cancer development
A, B Real-time qRT-PCR of key pro-tumorigenic cytokines related to CAC development in stable colon cancer cell lines transfected with the indicated plasmids (n = 3, biologically independent experiments, exact P values are shown in the figures). C The nuclear fraction experiment in HCT116 stable cell lines. Plasmids encoding ET-ITF2 (empty), FL-ITF2 (full length), or NT-ITF2 (N-terminal) were transfected into HCT116 cells and selected using G418 to generate stable cell lines. n = 2, biologically independent experiment. D The HCT116 stable cell lines which were transfected with ET-ITF2 or FL-ITF2 were treated with or without TNF for 8 h, and then cells were fractionated into cytoplasmic and nuclear extracts. n = 2, biologically independent experiment. C, D The extracts were immunoblotted by the indicated antibodies (C, D). In all immunoblot analyses, the data are representative of three independent experiments. All results are presented as means ± SEM. Statistical significance was determined by the unpaired two-tailed Student t test (A, B). ND not detected. Source data are provided in the Source Data file.
