Fig. 1: Development of synthetic sRNA systems for the knockdown of target genes.
From: Targeted and high-throughput gene knockdown in diverse bacteria using synthetic sRNAs
a Mechanism of synthetic sRNA-mediated translation repression. The sRNA scaffold and Hfq form a ribonucleoprotein complex and bind to a target binding region on the mRNA, resulting in the inhibition of protein translation by the ribosome. b Components for synthetic sRNA-based gene knockdown. TBR, target binding region. c Procedure taken to select sRNA candidates derived from Gram-positive bacteria. d Effects of various synthetic sRNA scaffolds and Hfq proteins on GFP fluorescence knockdown efficiency and cell growth of C. glutamicum. Abbreviations for Hfq: Bs, B. subtilis Hfq; Ec, E. coli Hfq; Lm, L. monocytogenes Hfq; Sa, S. aureus Hfq. Abbreviations for sRNA scaffolds: Arn, ArnA; Bts, BtsR1; Lhr, LhrA; Mic, MicC; Sco, Scr5239; Spr, SprX1; Rox, RoxS. *P < 0.0045, **P < 0.00091, determined by two-tailed Student’s t-test. P-value thresholds were adjusted using Bonferroni correction (corrected significance levels represented as α/m; α, original significance level; m, number of hypotheses). Error bar represents the mean ± standard deviation (SD; n = 3 biologically independent samples). NS, Not significant; arb. units, arbitrary units. Source data are provided as a Source Data file.
