Fig. 5: REV-I represses jejunal SR-B1 involving reduced transcriptional activity of NF-κB-p65.

a Jejunum expression of SREBP-1 detected by Western blotting; n = 3. b Comparison of expression of Srebf1 (which encodes SREBP-1) and its target genes in jejunum; n = 8. c Location of the conserved NF-κB p65 binding motif within human, rat and mouse SCARB1/Scarb1 promoters and nucleotide primers utilized in ChIP and qChIP. d Detection of cytoplasmic and nucleus NF-κB-p65 by Western blotting in the jejunum of indicated groups of mice, presented in Fig. 1; n = 4. The blot of NF-κB-p65 was stripped to re-probe for Lamin A. e Heatmap shows the comparison of intestinal inflammatory genes that are known to be regulated by NF-κB in the jejunum; n = 8 (see statistical analysis results of qRT-PCR in Supplementary Table 5). f Western blotting shows the relative expression of inflammasome components in the jejunum. n = 3. The blot of Pro-caspase-1 was stripped and re-probed for β-actin. ChIP shows the binding of NF-κB or RNA Polymerase II to the mouse Scarb1 promoter (g) (n = 6) but not the intron region (h) (n = 3) in the jejunum. qChIP shows the comparison of binding of NF-κB (i) (n = 6) or RNA Polymerase II (j) (n = 3) to mouse Scarb1 promoter in the jejunum. Statistical significance was evaluated by two-sided one-way ANOVA with Dunnett’s post hoc test (compared with the HFD group). *p < 0.05, **p < 0.01, ***p < 0.001. Data are presented as mean ± SD. Source data are provided as a Source Data file.