Fig. 7: FLP-5/DMSR-1 signaling mediates speed elevation to promote glocal search.

Impact of neuropeptide-affecting mutations on the speed of starved animals at 25 °C (a) and after a tonic optogenetic FLP stimulation reported as in Fig. 5 (b, c, d). Impact of flp-5 mutation, over-expression, and rescue/over-expression with Pmec-3::flp-5 transgene expressed in FLP (e) and of loss-of-function mutations affecting FLP-5 receptors (h) on the speed of starved animals at 25 °C. flp-5 transcriptional reporter quantification comparing mean intensity (f) and fraction of FLP neurons with detectable signal (g). Food drop assays reported as in Fig. 2 showing reduced food-reaching performances in flp-5; dmsr-1 (i, j). Impact of gain-of-function (gf) and loss-of-function (lf) mutations in egl-6, as well as FLP-5 receptor over-expression in egl-6-expressing cells, revealing that EGL-6, DMSR-1a, DMSR-1b and DMSR-7 have a similar inhibitory effect on the speed of starved animals at 25 °C (k). Opposite impact of broad DMSR-1 overexpression in dmsr-1-expressing cells or AVA-specific overexpression, respectively, on the speed of starved animals at 25 °C (l). Data as average ± s.e.m. with number of independent assays, n, indicated on each graph (a–e, h, j, k, l). *p < 0.05 and **p < 0.01 versus 15 °C Fed condition or respective N2 control; #p < 0.05 and ##, p < 0.01 versus the indicated condition by Bonferroni posthoc tests. +p < 0.05 and ++p < 0.01 versus 15 °C Fed or indicated condition by Fisher’s exact test for contingency comparisons. ns not significant. Source data are provided as a Source Data file.