Fig. 4: The largest orthogonal subsets of the CCmax library. | Nature Communications

Fig. 4: The largest orthogonal subsets of the CCmax library.

From: A multiplexed bacterial two-hybrid for rapid characterization of protein–protein interactions and iterative protein design

Fig. 4

A Design of the CCmax library. Using several iCipa variants 22 sets comprised of 18,491 interactions were designed and orthogonal sets of orthogonal interactions with a given orthogonality gap were identified (Supplementary Data 6). B Number of on-target orthogonal interactions per orthogonal subset at orthogonality gap of 1.0 Interaction Score. Between two and fifteen on-target, orthogonal interactions were obtained per subset. C The largest orthogonal subset contains fifteen on-target interactions across 318 tested pairs. Grey boxes represent designed on-target interactions. D The largest orthogonality gap per number on-target interactions in a set. E iCipa’s agreement with the Interaction score (R2 = 0.429). The black line is a linear model predicting interaction scores from iCipa predictions. Source data are provided as a source data file.

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