Fig. 2: Exposure to fluctuated temperature aggravates sarcopenia in mice.
From: Augmented temperature fluctuation aggravates muscular atrophy through the gut microbiota
a Experimental scheme. 12-month-old male mice were exposed to room temperature (RT), low temperature (LT) and fluctuated temperature (FT) for 12 weeks. b–d Determination of exercise capacity by conducting rotarod test (b), grip test (c) and treadmill test (d). n = 9 biologically independent animals per group. e, f Weight and representative pictures of dissected quadriceps femoris, tibialis anterior, gastrocnemius and soleus muscles. n = 9 biologically independent animals per group. g–i Representative images of HE staining of gastrocnemius muscle cross-section (h), quantification of mean cross-sectional areas (g) and distribution of muscle fibers (i). Bar = 80 μm. n = 5 biologically independent animals per group. j, k Representative images of succinate dehydrogenase (SDH) staining of tibialis anterior muscle cross-section (j) and quantification (k) of stained muscle fibers. Bar = 125 μm. n = 5 biologically independent animals per group. l Expression levels of Myostatin, Atrogin1, Musa, and MuRF1 measured by qPCR. n = 5 biologically independent animals per group. Data presented are mean ± s.e.m. One-way ANOVA test for multiple comparisons with Tukey’s test for post hoc corrections. Source data are provided as a Source Data File.
