Fig. 5: Fluctuated temperature treatment leads to mitochondrial dysfunction in muscle.
From: Augmented temperature fluctuation aggravates muscular atrophy through the gut microbiota
a, b Representative transmission electron micrographs (TEM) of intermyofibrillar (IMF) mitochondria from gastrocnemius of RT, FT, FMTrt, and FMTft mice (a) and quantification (b) of relative mitochondria area. n = 5 biologically independent animals per group. c Determination of ATP contents of muscle tissue, protein levels were used for adjustment. n = 5 biologically independent animals per group. d Determination of ROS level in digested muscle cells through DCF probe. n = 5 biologically independent animals per group. e, f Determination of mitochondrial membrane potential (MMP) by JC-1 probe and TMRE probe in isolated mitochondria from muscle tissue. n = 5 biologically independent animals per group. g Quantification of mtDNA/nuDNA ratio. n = 5 biologically independent animals per group. h Expression levels of mitochondrial biogenesis-, dynamics-, and mitophagy-related genes by qPCR. n = 5 biologically independent animals per group. i, j Western blot (i) of mitochondrial biogenesis-, dynamics-, and mitophagy-related biomarkers and quantification of the bands in the gels (j). n = 3 biologically independent animals per group. Data presented are mean ± s.e.m. One-way ANOVA test for multiple comparisons with Tukey’s test for post hoc corrections. Source data are provided as a Source Data File.
