Fig. 1: Mapping and validation of Tvp23b in chipotle phenotype.
a Percentage of body weight lost on day 7 of DSS treatment plotted per genotype [REF Tvp23b+/+ (n = 8); HET Tvp23b+/chipotle (n = 13); VAR Tvp23bchipotle/chipotle (n = 2)]. b Manhattan plot showing P values of association between chipotle and mutations identified in chipotle pedigree, calculated using an additive model of inheritance. The −log10 P values (y-axis) are plotted versus the chromosomal positions of the mutations (x-axis). Horizontal red and purple lines represent thresholds of P = 0.05 with or without Bonferroni correction, respectively. P values for linkage of mutation in Tvp23b with the chipotle DSS phenotype are indicated. c Weight loss analysis of mice of Tvp23b+/+, Tvp23b+/−, and Tvp23b−/− after 1.4% DSS treatment (n = 5 independent mice for all groups) from CRISPR/Cas9 targeted mice. (**P = 0.0015, ***P < 0.0001) d, e Disease activity index and colonic length of individual mice after 7 days of DSS challenge (n = 5 independent mice for each group). (*P = 0.043, **P = 0.015, ****P < 0.0001). f Representative Hematoxylin and Eosin staining of Tvp23b+/+ and Tvp23b−/− colons after 7 days of DSS treatment. Scale bars: 50 μm. g Disease activity index of mice 11 days post oral infection with C. rodentium (n = 8 independent mice for each group, **P = 0.0064). h, i Streptomycin-resistant colony forming units of Feces (***P = 0.0006) and Cecal content (***P = 0.0003) after oral C. rodentium infection. Data are expressed as means ± s.d. and significance was determined by two-way ANOVA with Dunnett’s multiple comparisons (c), one-way ANOVA with Dunnett’s multiple comparisons (d, e), Mann–Whitney test (g, h, i). Data are representative of at least three independent experiments (c–i).
