Fig. 4: Abnormal goblet cell morphology and mucus production. | Nature Communications

Fig. 4: Abnormal goblet cell morphology and mucus production.

From: Trans-Golgi protein TVP23B regulates host-microbe interactions via Paneth cell homeostasis and Goblet cell glycosylation

Fig. 4

a, b Representative Alcian-Blue staining and electron microscopy of distal colon from Tvp23b+/+ and Tvp23b−/− animals. c, d Representative PAS stained colonoids (20×) from Tvp23b+/+ and Tvp23b−/− animals. Quantitation of PAS+ vacuole size from colonoids (n = 80 and n = 73 individual Goblet cells derived from 3 independent Tvp23b+/+ mice and 3 Tvp23b−/− mice, ****P < 0.0001). e, f Ex vivo mucus thickness and growth rate from Tvp23b+/- and Tvp23b−/− colons (n = 10 independent mice per group **P = 0.0017, ***P = 0.0002). g Ex vivo induction of mucus by lipopolysaccharide in Tvp23b+/− and Tvp23b−/− colons (n = 5 independent mice per group, *P = 0.0149, **P = 0.0091). Data are expressed as means ± s.d. and significance was determined by unpaired student t-test (d) one-way ANOVA with Dunnett’s multiple comparisons (e, f) and two-way ANOVA (g).

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