Fig. 5: The systemic comparison of light- and Ang1- activated Akt1 downstream signaling.
From: An optogenetic-phosphoproteomic study reveals dynamic Akt1 signaling profiles in endothelial cells
a Light- and Ang1-activated Akt1 signaling demonstrates an opportunity dissecting the usage of individual P sites for Akt1 and Ang1 signaling. b maSigPro analysis classified differential temporal profiles from time-course datasets into four clusters (mC1-mC4), for which the log2 fold-changes Fold changes (FC) values (10/20/30 min compared to 0 min) in each time were visualized in nested boxplots (the number of P-sites for mC1-mC4 clusters are indicated above the boxplots; center line, median; box limits, upper and lower quantiles; whiskers, min to max values). c P-site-specific enrichment analysis for known kinase substrates among mC1-mC4 (all P values < 0.05). In the mC3&4 circle, the asterisk following GSK3B denotes that only GSK3B is enriched from mC4, whereas other kinases are enriched from mC3. Error bar denotes SD. d Examples of P-sites with their possible, annotated kinase enriched based on OmniPath and PSPdb (n = 2 biologically independent samples, histogram bars denote mean). Source data are provided as a Source data file.
