Fig. 4: ExPRESSO enables multiplexed proteomic assessment of archival human brain sections at subcellular resolution.
From: Expanded vacuum-stable gels for multiplexed high-resolution spatial histopathology

a MIBI imaging of an ExPRESSO-processed human brain tissue section from the middle frontal gyrus. Top: representative linear acquisition of 16 FOVs, each one with a size of 400 × 400 μm (physical measurements). Images include markers for neurons (MAP2), vessel lumen and BBB leakiness (Albumin), astrocytes (GFAP and GlnSyn), vessels (GLUT1), microglia (Iba1), and nuclei (Histone H3). Bottom: enlarged views of the gray matter (left; GFAP and GlnSyn high) and white matter (right; GFAP and GlnSyn low). Scale bars (the ExPRESSO-MIBI scale bars indicate the pre-expansion dimensions based on a 3.7-fold expansion): 50 μm. b MIBI imaging of an ExPRESSO-processed human hippocampus tissue section. Middle: Representative tile acquisition of nine FOVs, each one with a size of 400 × 400 μm (physical measurements). Images include markers for pre-synapse (Synaptophysin), astrocyte projections (GFAP), nuclei (Histone H3), microglia (Iba1), vessel lumen, and BBB leakiness (Fibrinogen), vessels (GLUT1), muscularized vasculature (SMA). Left and right: Enlarged views of the blood-brain barrier (BBB). The BBB images at the top include the markers in the middle image and the BBB images at the bottom include markers for basement membrane (Collagen IV), endothelial cells (CD105(Endoglin), and vWF), astrocytes (GFAP), neurons (MAP2), pre-synapse (VAMP2), and neurons with oxidative stress (8OHG). Scale bars (the ExPRESSO-MIBI scale bars indicate the pre-expansion dimensions based on a 3.7-fold expansion): 50 μm (middle) and 10 μm (left and right). c Representative MIBI images of cellular and non-nuclear components in the human brain. From left to right, images highlight microglia, vessels, astrocytes, and BBB. Vessels are observed as transversal (top) and longitudinal (bottom) sections. Scale bars (the ExPRESSO-MIBI scale bars indicate the pre-expansion dimensions based on a 3.7-fold expansion): 25 μm.