Fig. 5: Difference between WT and acetylmimetic K39Q nuclear magnetic resonance parameters.
A Average differences (means) in longitudinal relaxation rate R1 (top), transversal relaxation rate R2 (middle), and heteronuclear 15N{1H} NOE (bottom) between the experimental values at 500 MHz for the reduced forms of K39Q and WT Cytc, plotted as a function of the residue number (n = 2). Each difference was calculated from a set of relaxation parameters measured on two biologically independent samples for WT and K39Q Cytc. Raw data is represented in Supplementary Fig. 8. A scheme of the secondary structure elements is included at the top and the mutated position is indicated with dashed lines. B Ribbon structure of K39Q Cytc colored according to the difference in its dynamic properties from blue (decrease) to red (increase) compared with WT protein. Undetectable or overlapping backbone resonances are in gray. Source data are provided as a Source Data file.
