Fig. 6: Inhibition of PITPNA in isolated human islets disrupts subcellular localization of PtdIns-4-P to the TGN.
From: Restoration of PITPNA in Type 2 diabetic human islets reverses pancreatic beta-cell dysfunction
a, b Immunostaining for INSULIN and Ptdlns-4-P, and quantification of the intensity of Ptdlns-4-P in isolated human islets after lentiviral-mediated inhibition of PITPNA (sh-PITPNA, n = 7) or treatment with control lentivirus (sh-Ctrl, n = 5). P = 0.0041. Scale bar = 20 µm. c, d Quantification of GOLGIN97 and GOLPH3 colocalization after immunostaining of isolated human islets after lentiviral-mediated inhibition of PITPNA (sh-PITPNA) (n = 10) or treatment with control lentivirus (sh-Ctrl) (n = 23). P < 0.0001. Scale bar = 10 µm. e Quantification of phosphatidylinositol-phosphate (PtdIns or PIP) species in isolated human islets after lentiviral-mediated over-expression of PITPNA (OE-PITPNA) or treatment with a control lentivirus (OE-Ctrl) (n = 3). Results normalized to total cellular PtdIns (PI). PPIP34:0 = 0.3085, PPIP34:1 = 0.2069, PPIP34:2 = 0.9604, PPIP36:0 = 0.0432, PPIP36:1 = 0.0703, PPIP36:2 = 0.0646, PPIP36:3 = 0.05, PPIP36:4 = 0.0788, PPIP38:2 = 0.0815, PPIP38:3 = 0.069, PPIP38:4 = 0.0394, PPIP38:5 = 0.3604, Pflod change = 0.045. f Quantification of phosphatidylinositol-phosphate (PtdIns or PIP) species in isolated human islets after lentiviral-mediated inhibition of PITPNA (sh-PITPNA) or treatment with a lentivirus expressing an shRNA control (sh-Ctrl) (n = 4). Results normalized to total cellular PtdIns (PI). PPIP34:1 = 0.6432, PPIP34:2 = 0.958, PPIP36:0 = 0.793, PPIP36:1 = 0.6891, PPIP36:2 = 0.9853, PPIP36:3 = 0.9284, PPIP36:4 = 0.3006, PPIP38:2 = 0.9485, PPIP38:3 = 0.9601, PPIP38:4 = 0.8663, PPIP38:5 = 0.9815, PPIP40:4 = 0.8955, PPIP40:5 = 0.8219, Pflod change = 0.0284. Data are presented as mean values ± SEM for (b), (d), (e), (f). *P < 0.05, **P < 0.01, ***P < 0.001, and n.s. denotes not significant. Two-tailed unpaired Student t-test were used in for (b), (d), (e), (f). All primary source data are reported in the Source data file.
