Fig. 7: The TSM remodeling mediated by the photothermal effects of UHHTN/DOX nanorobots in vivo.
a HE staining and TUNEL analysis of TNBC spinal metastasis were performed 20 days after nanorobot injection at a DOX dosage of 5 mg kg−1 once every four days. Scale bar, 10 μm in HE staining; Scale bar, 50 μm in TUNEL analysis). b Immunofluorescence showing the proportion of CAFs (α-SMA+green signal/CD31-red signal) and TAMs (F4/80 green signal) in tumors in different groups at a DOX dosage of 5 mg kg−1 once every four days after 20 days therapy. Scale bar, 50 μm. α-smooth muscle actin, α-SMA; cancer associated fibroblast, CAF;Tumor-associated macrophage, TAM; 4’,6-diamidino-2-phenylindole, DAPI. c Immunofluorescence showing the expression of collagen I (green signal) and fibronectin (green signal) in tumors of different groups at a DOX dosage of 5 mg kg−1 once every four days after 20 days therapy. Scale bar, 50 μm. d Quantitative mean optical density (MOD) results from TUNEL analysis fluorescence in tumor tissues of different groups after 20 days therapy. Data are presented as the mean ± s.d. Statistical analysis was performed by two-tailed Student’s t test (n = 3 replicates; **P < 0.05, ***P < 0.001, ****P < 0.0001). e Quantitative MOD results from α-SMA, CD31, F4/80, collagen I and fibronectin fluorescence in tumors of different treatment groups after 20 days therapy. Data are presented as the mean ± s.d. Statistical analysis was performed by two-tailed Student’s t test (n = 3 replicates; **P < 0.05, ***P < 0.001, ****P < 0.0001). f Real-time microdistribution in MDA-MB-231 tumors at 30, 60, and 120 min after UHHTN/Cy5.5 nanorobot and AuNS@SiO2/Cy5.5 by intravenous injection at a Cy5.5-equivalent dose of 0.5 mg kg −1. Scale bar, 100 μm. Source data for the figure are provided within the Source Data file.
