Fig. 4: Mechanism of Lipid II transglycosylation.
From: Structural basis of peptidoglycan synthesis by E. coli RodA-PBP2 complex
a RodA active site with RodA shown as green ribbon and PBP2 shown as blue ribbon, residues of interest represented as sticks. Cut-out from Weblogo plot, as in Supplementary Fig. 6, shown for residues of interest, residues are represented as sticks. Cryo-EM density in cavity A proposed to correspond to Und-PP displayed as a yellow surface. b Functional analysis of RodA-PBP2 GT activity using in vitro Lipid II polymerization assay, monitoring the effect of different mutations on selected residues. Source data are provided as a Source Data file. c Schematic representation of the mechanistic model for the GT reaction between two Lipid II molecules by RodA viewed from the periplasmic side of the membrane. The active site Asp262 is highlighted in green.
